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Isolation of genomic and cDNA, construction of genomic libraries, and methods of introducing foreign DNA into host cells (transformation, electroporation, microinjection).
Biotechnology (U. Satyanarayana) ├── Part I: Chemical Basis of Life (Biomolecules, Enzymes, Energetics) ├── Part II: Molecular Biology and General Biotechnology (DNA, RNA, Replication) ├── Part III: Recombinant DNA Technology (Cloning, Vectors, PCR, CRISPR) ├── Part IV: Agricultural & Environmental Biotechnology (Transgenic Plants, Bioremediation) └── Part V: Industrial & Medical Biotechnology (Fermentation, Antibiotics, Gene Therapy) Core Topics Covered 1. Molecular Biology and Genetic Engineering
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Modern vaccine development technologies, including mRNA platforms.
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History, scope, and multidisciplinary nature. Satyanarayana) ├── Part I: Chemical Basis of Life
Methods for creating transgenic mice, cattle, and fish, alongside the production of therapeutic proteins (molecular farming).
Production of recombinant pharmaceuticals like insulin, growth hormones, and interferons.
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Provides case studies and bridges the gap between basic theory and medical applications.
Fermentation technology, bioreactor designs, and downstream processing.